Catalog Number :Rs-1948R
Quantity size : 0.2ml---dilute with pH 7.4 0.01 M PBS or antibody diluent
Background: Wnt proteins form a family of highly conserved secreted signaling molecules that regulate cell-to-cell interactions during embryogenesis. Wnt genes and Wnt signaling are also implicated in cancer. Insights into the mechanisms of Wnt action have emerged from several systems: genetics in Drosophila and Caenorhabditis elegans; biochemistry in cell culture and ectopic gene expression in Xenopus embryos. Many Wnt genes in the mouse have been mutated, leading to very specific developmental defects. As currently understood, Wnt proteins bind to receptors of the Frizzled and LRP families on the cell surface. Through several cytoplasmic relay components, the signal is transduced to b-catenin, which then enters the nucleus and forms a complex with TCF to activate transcription of Wnt target genes.
Also known as: wingless-related MMTV integration site ; hWNT ; hWNT; Protein Wnt ; Protein Wnt; Wingless type MMTV integration site family member ; Wnt ; WNT protein precursor; WNT protein precursor.
Specificity : · anti- WNT is a rabbit monoclonal antibody unconjugated
· specific for WNT of hu, mo, rat, cow, Rb
· epitope mapping near the C- terminus of WNT of human, mo, rat
· use for western blotting, elisa, immunoprecipitation and immunohistochemistry
· Protein A affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt: 42kDa
Application:· Western blotting 1:100-500
· Immunohistochemistry 1:100-500
· ELISA 1:500-1000
· Optimal working dilutions must be determined by the end user.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When
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